Absent sites are indicated in italic in enzyme chooser windows (Graphic and Restriction Maps) It is now possible to fully resize Sequence windows A menu entry to go to Serial Cloner Youtube Channel (tutorial) A menu entry to go to Serial Cloner Forum Added a shortcut to 'Copy Non Formatted' A button to quickly select a full linear insert in the 'Build a Construct' window 'Anchor' and 'Core' domains of Primers are now recognized as Features in Sequence Windows A shortcut (S) to quickly show or mask Features (in the Features tab of Sequence Windows) A toggle button to highlight differences in aligned proteins Display Features in Protein Align (Local Align) Inherit Features when copying/Pasting Protein sequences Non formatted copy of Protein sequence (Copy Moreā¦ Menu) A rapid switch between a DNA and Protein Align window Inherit Features when reverse-translating a DNA sequence Scan for peptide Features in Protein Sequences A button/menu to choose particular sites to be displayed in Restriction maps. An option in the Restriction Map window to only translate nucleotides that are in uppercase An option in the Preferences to use the Codon code from Codon Usage tables as a Genetic code used for translation An option in the Preferences to select pK value tables to use for pI estimation MacOSX version now distributed as a signed application (Mountain Lion Gatekeeper compatibility) Estimates Isoelectric point of protein sequences (DNA and Protein Sequence Window) Insertion point position when editing a protein sequence Error in definition of PreScission site Slow down under certain Windows install due to autosaving Bug in "show info" toggle under Windows and Linux Detection of overlapping Primers in PCR a preference to limit or not the width of Seq & Prot windows (see the available contextual help window to get more details on new functions) It also allows to generate sense and antisense sequences to be obtained after bi-sulfite conversion. Serial Cloner 2.5 now allows to import codon frequency tables from the internet using a Web interface. It is also posible to send directly BLAST request at the NCBI and obtain the result inside a Web interface. You will also find a Restriction Enzyme library management interface, Additional tools, like a web browser for direct import of NCBI and EMBL entries, a virtual cutter to prepare restriction analysis or a silent restriction map generator to find how to introduce restriction sites without modifying the translated peptide are also provided. Features are now visible when aligning locally. Finally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server. An additional interface allows easy Gateway(tm) cloning for both BP and LR reactions. Just select, blunt if you need, and click the Ligate button. Finally, you can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. shRNA constructions based on pre-defined scaffolds are also automated. In conclusion, Serial Cloner is a very user-friendly and powerful Molecular biology tool.PCR-based fragment or synthetic adaptors. Fragments obtained by PCR, shRNA synthesis, can be easily assembled by selecting between the restriction sites.We can also ligate fragments using the ligate button.Also, to create new PCR based fragments or synthetic adaptors. We can use either the graphics window or the text-based site usage windows, which makes it very easy to quickly extract a sub-sequence in a selection or in any restriction sites.There is a web browser tool within the software to allow direct import of NCBI and EMBL.Sequence features are visible on the sequence map.They can be automatically found by using the scan feature. Features can be manually entered or imported.The fully graphical map makes it easy to extract and select a fragment or even show single, double, or multiple cutters in a single window.All the tools required to analyze the sequence and modify if any changes needed are available in a single window making it very easy to use.The software comes with a high-quality graphics display and has a very user-friendly interface to the front end, which makes the interface very intuitive. They are processed both in sequence and as a graphic map. Since version 2.0 of the software, it also handles Annotations and features. It can read and write files in several formats, making it portable across multiple users who use different Molecular Biology tools. It is available free for download and can be used across multiple Operating Systems, including Windows, Mac, and Linux. Serial Cloner is a Molecular Biology Tool which is a front end to help with DNA Cloning, Sequence Analysis, and Visualization.
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